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Wednesday, March 28, 2007
Hobby Microscopes
Hobby Microscopes are the instruments available for any budget. They are a good for the young people as well as old. There are Hobby Microscopes that are used for any hobbyists you can only imagine, starting from coin and stamp collectors, geologists, to shell collectors and a wide range of other hobby applications.Hobby Microscopes can be of any type: compund microscope, stereo microscope, have any body type, or even microscope lighting system. Hobby microscopes can be found in homes as well as at schools. With Hobby microscopes you'll be able to see the invisible world in living color. They can used to observe slides. Small size and light weight of the Hobby Microscopes makes them easily portable.
Posted by OpticsPlanet at 2:18 PM Read Article 0 comments 

Tuesday, March 27, 2007
Gemology microscopes
Gemology microscope is the most-used instrument among gemologists, next to refractometer and a loupe. Gemology microscopes are used primarily for magnification of internal and external features of gemstones.
Usually gemologists use binocular microscopes with zoom capabilities. These binocular zoom microscopes are the most expensive pieces of identification equipment used in the standard gemological laboratory.
Any good gemology microscope must be equiped with lightfield and darkfield illumination. Inclusions will stand out bright when using darkfield illumination technique, which appears to be the most used type of illumination in gemology.
Monday, March 26, 2007
Industrial Microscopes
When choosing a microscope, your goal is to get the right microscopic tool for the right application. Since microscopes are used in a variety of fields, it means that there are many types of microscopes and microscope accessories.
As for industrial microscopes, they are used primarily in assembly work or quality control. From its name you can guess that they are used to inspect materials and finished products. Most often stereo microscopes with special stands, illumination systems and digital imaging systems are used for industrial applications. The more common industrial microscopes are: measuring microscopes, metallurgical microscopes, shop microscopes, and geological microscopes.
Thursday, March 22, 2007
Metallographs
Metallograph is an optical microscope equipped with a camera for both visual observation and photography of the structure and constitution of a metal or alloy.
Metallography is the art and science at the same time of preparing a metal surface for analysis by grinding, polishing, and etching to reveal microstructual constituents. After preparation, the sample can be easily analyzed using optical microscope or electron microscope. Any skilled technician is able to identify alloys and predict material properties, as well as processing conditions by metallography alone.
Metallurgical Microscopes
Metallurgical Microscopes can be in the form of: upright metallurgical microscopes, inverted metallurgical microscopes, and portable field metallurgical microscopes. The most common configurations of metallurgical microscopes are student, benchtop and research. Student microscopes are the smallest and least expensive type of microscope. They can provide an advanced techniques and documentation even though they are for student use. Benchtop microscopes are used in various industries like textiles and animal husbandry. Research microscopes are huge. They may use multiple cameras, large specimens, and the widest range of simultaneous techniques.
Often metallurgical microscopes are used for measuring thin films and electroplating coatings, inclusions, surface defects, and grain size.
Metallurgical microscopes can be of many types of technologies. The most common metallurgical microscopes are acoustic or ultrasonic microscopes which can be used to examine delimitations, cracks and other anomalies nondestructively and inverted microscopes which are useful for flat polished metallurgical, ceramic, or optical samples. Metallurgical microscopes can come in one of many types of eyepiece styles. These include monocular microscopes, binocular microscopes, trinocular microscopes, or dual head.
Live Blood Analysis Microscopes
Two drops of blood under a specialized live blood analysis microscope reveals anomalies in the blood that relate to deficiencies in nutrients, dysfunctional bodily systems, toxins and dysbiosis of the human body. Since the 1920's scientists have been studying the hidden secrets of our blood. A special lens inside the microscope projects an intimate view of your living blood onto a television screen by way of a video microscopy system. The camera is hooked up to a device enabling to take photographs of a patient's blood condition before and after treatment. A microscope view of one drop of blood is projected onto a video monitor, showing your "internal" nutritional environment. Live blood Microscopy was first used in medicine for diagnosing infections, and now the Live blood Microscopy is gaining popularity, especially with naturopaths and holistic doctors.
Friday, March 16, 2007
Research Microscopes
Research microscopes are big. A modern research microscope will weigh in the range of 66 lb to 110 lb. This mass is composed of big optical systems, big mechanical systems and lots of electronics. These microscopes can also use multiple cameras, large specimens and the widest range of simultaneous techniques. Most have built in computers to control digital microscope cameras and other functions including focus. If you are doing particularly demanding work or if you do a lot of documentation then you definitely need one of them. While they are expensive they can do what no other microscope can do.
Hoffman Modulation Contrast
The Hoffman modulation contrast (HMC) microscope is another method how transparent, or close to it, objects can be visualized. Hoffman modulation contrast accentuates phase gradients within the sample, and displays them in the image as levels of gray modulated lighter or darker than an average background gray. A special filter, the modulator, is placed in the objective and a special aperture. It is available as new standard equipment or for upgrading compound microscopes. The modulator filter is constructed so as to have three regions of different neutral densities, ranging from low to a high attenuation. The condenser slit is adjusted so that light transmission through the slit falls on the gray region of the modulator. HMC offers, at moderate cost, virtually all of the capabilities of the other contrast systems, plus important additional advantages. Hoffman Modulation Contrast System is a cost effective upgrade to 3-dimensional appearing images for both the inverted and upright compound microscope.
Laboratory Microscopes
A laboratory microscope is the general term for any type of a microscope that is designed for use in laboratories. Laboratory microscopes can be upright or inverted in design, and in most cases are designed large, sturdy and modular so they can withstand the wear and tear of the laboratory and handle a variety of samples and tasks. Stereomicroscopes are also widely used in laboratories. Stereomicroscopes are especially beneficial for sample preparation, bulk sample analysis, dissection, and whenever large working distances, low magnification and large depth of field are required.
Tuesday, March 13, 2007
Differential interference contrast
Differential interference contrast (DIC) microscopy is a beam-shearing interference technique in which the reference beam is sheared by a minuscule amount. This system produces a monochromatic image that effectively displays the gradient of optical paths for high and low spatial frequencies present in the specimen. Those regions of the specimen where the optical paths increase along a reference direction appear brighter (or sometimes darker), while regions where the path differences decrease appear in reverse contrast. As the gradient of optical path difference grows steeper, image contrast is usually dramatically increased.
As with Phase Contrast Microscopes, DIC transforms the phase shift of light, induced by the specimen refractive index, into detectable amplitude differences. An advantage of interference-derived contrast is that an object will appear bright against a dark background but without the diffraction halo associated with phase contrast.
Differential interference contrast microscopes are actually microscope interferometers in that they generate contrast within the specimen by exploiting phase differences between a specimen light ray and a reference ray.
Monday, March 12, 2007
Fluorescence Microscope
A Fluorescence Microscope is a light microscope used to study properties of organic or nonorganic substances using the phenomena of fluorescence and phosphorescence instead of reflection and absorption. These microscopes became an important part in the field of biology and medicine. The fluorescence technique has made it possible to identify cells and cellular components with a high degree of specificity.
In fluorescence microscopy, the sample to study is itself the light source. The technique is used to study specimens, which can be made to fluoresce. The fluorescence microscope is based on the phenomenon that certain material emits energy detectable as visible light when irradiated with the light of a specific wavelength. The sample can either be fluorescing in its natural form like chlorophyll and some minerals, or treated with fluorescing chemicals.
Fluorescence microscopy is a rapid expanding technique, both in the medical and biological sciences.
Sunday, March 11, 2007
POLIRAZED LIGHT MICROSCOPES
Polarized Light Microscope is a microscope that utilizes polarized light to form a highly magnified image of an object. Polarizing microscopes play an important role in crystallography, petrography, microchemistry, and biology. Although all light microscopes compare poorly with electron microscopes with respect to image resolution, polarized light microscopes have the unique ability to deliver information about the submicroscopic structure of the objects being examined. They also have the advantage of being relatively nondestructive, and may be used safely with living cells.
Polarized light microscopy positions two polarizing filters, called polarizers, in the light path. The polarizing filters are very similar to those found on polarizing sunglasses. Ideally, both polarizers will be rotatable. One polarizer is positioned before the sample, and the second (called the analyzer) is located between the back focal plane of the objective and the eyepieces (or camera).
There are two traditional modes of use for the polarizing microscope, the orthoscopic mode and the conoscopic mode. In the orthoscopic mode, the ocular projects an image of the specimen, as in conventional microscopy. The conoscopic mode is used to characterize crystalline specimens.
OBLIQUE ILLUMINATION
Specimens that are nearly transparent and colorless may be almost invisible when viewed in the stereo microscope using traditional transmitted brightfield illumination techniques. This occurs because light diffracted by minute specimen detail is a quarter-wavelength out of phase with direct light passing through the specimen when both are recombined in the intermediate image plane, a classical phenomenon that seriously reduces contrast in brightfield images.
Oblique illumination is similar in many aspects to the darkfield technique except that, instead of the specimen being lighted from all directions at oblique angles, light is projected from only a single azimuth. Simple diascopic bases are often equipped with a tilting mirror that can be adjusted to provide a certain degree of oblique illumination, but the light is not easily controlled and does not provide a uniform field of view. More complex microscope stands (or bases) have additional control possibilities, including tilting mirrors that are not restricted to a single axis and sliding mirror assemblies that can be inserted and removed from the light path.
Any of these techniques can provide acceptable to excellent results on a wide variety of specimens.
Wednesday, March 07, 2007
Darkfield Microscopes
Darkfield microscopes employ a special contrast enhancing technique known as dark field illumination to produce beautiful images of normally difficult-to-observe biological specimens. Under phase contrast conditions, the light coming through the specimen is shifted into two beams, one slightly out of phase with the other. This gets a little complicated to explain easily, but as far as equipment concerns, you need two matched items in order to get phase contrast. Similar to the phenomenon of being able to see stars at night but not during the day, darkfield illumination is most often used with samples that are not easily imaged against a light background, and results in samples that appear bright against a dark background. Darkfield microscopy is a very common and economical contrast technique to equip a microscope with, especially for low magnification use. The dark field microscope has found considerable use within the the field of live blood microscopy (sometimes referred to as live cell microscopy or darkfield analysis of live blood). However, dark field illumination is just as useful on an inverted metallurgical microscope, as well as for diatoms, small aquatic organisms and many other biological and metallurgical samples.
Phase Contrast Microscopes
The phase contrast microscopes are widely used for a variety of applications such as molecular and cellular biology research, clinical and medical diagnosis of health conditions, living tissue culture viewing, microbiological research, dog breeding, horse breeding, animal breeding, infertility treatments, analysis of spermatoza in semen, sperm cell motility, sperm count, sperm morphology, mold and mildew detection and abatement, mold spore count, mold spore identification, stachybotrys (toxic black mold, stack mold) treatment, aspergillus mold identification, environmental air pollution monitoring, viewing of human squamous cheek cells, dust mite control, and asbestos testing. It is a type of light microscopy that enhances contrasts of transparent and colorless objects by influencing the optical path of light. The phase contrast microscope is able to show components in a cell or bacteria, which would be very difficult to see in an ordinary light microscope. Phase contrast microscopes, or phase contrast microscopy, employ a special illumination technique known as phase contrast to improve the contrast in unstained biological samples without significantly sacrificing image resolution. Similar to darkfield illumination, phase contrast is ideal for use with transparent samples that seem to blend into a light background, and results in specimens that contrast from a darker background. The phase contrast effect is achieved by superimposing a circular phase annulus located in the sub-stage condenser with a corresponding circular phase ring located in the back focal plane of the objective lens. Specimens subjected to phase contrast illumination often appear to be surrounded by halos, and appear against a grayish background if used with plain white light, and appear against a green background if used with a green filter in the light path. Phase contrast is one of the most, if not the most, commonly used biological contrast enhancing technique today. Phase contrast microscopy can be performed on upright microscopes and inverted microscopes, and is especially useful in the study of living cells and tissues, aquatic and other microorganisms, microbiological features such as cell nuclei, glass fragments and fibers, mineral fibers such as asbestos, bacterial cultures and more.
Monday, March 05, 2007
Brightfield microscopes
Brightfield microscopes are what most people visualize when they think of a microscope, where the image is formed due to the absorbing properties of the imaged objects. Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. Brightfield microscopy is when the illumination is unaltered both before and after hitting the sample. The technique is best suited for utilization with fixed, stained specimens or other kinds of samples that naturally absorb significant amounts of visible light. . Images produced with brightfield illumination appear dark and/or highly colored against a bright, often light gray or white, background. Bright Field microscopes can be upright microscopes or inverted microscopes in design and are most effective when used with samples that are stained, or samples that naturally contrast with the background or mounting medium in color, morphology or both
Thursday, March 01, 2007
Transmitted Light microscope
Transmitted Light microscopes are the most common type of a compound microscope. These microscopes are the exact type used in biology classrooms and doctors' offices, and other places. The object is normally placed on a clear glass slide, and light is transmitted though the object.
Transmitted light is occasionally used for transparent and translucent materials. For some low-magnification work, external, oblique illumination can be reflected off the sample into the objective.
