Density Gradient Centrifugation

Density gradient centrifugation is a method which scientists use to separate intact organelles from a cell. Each type of organelles in a cell has an specific density and based on that density you can separate a certain type of organelle from the others. In In the life sciences, a special technique called density gradient separation is used for isolating and purifying cells, viruses and subcellular particles. Variations of this include Isopycnic centrifugation, Differential centrifugation, and Sucrose gradient centrifugation . A blood donation technique called Pheresis involves density gradient separation. For this type of centrifugation method you may need a refrigerated centrifuge, especially if you are using this for blood work. Not all Density Gradient Centrifugation methods require a refrigerated centrifuge. Some cellular work does not require refrigerated centrifugation. You may be able to use a non refrigerated centrifuge. The Beckman Coulter Centrifuge, Allegra X-12 and Allegra X-12R are refrigerated and non refrigerated models. Again with both of these models you will need to select a rotor and tube adapter separately. Don't forget your centrifuge tubes 2.0ml tubes and 15ml tubes are the most commonly used sizes.