MicroscopesBlog: Expert's reviews, news, notes on Microscopes, Centrifuges, Lab Coats, Pipettes, Lab Safety, & more Lab Products!

Friday, July 31, 2009

Southern Blot

A southern blot is a laboratory technique used in molecular biology to detect a specific DNA sequence. Before performing a southern blot, DNA is digested by restriction endonucleases which produce DNA fragments. These DNA fragments are then separated in an agarose gel and transferred to a southern blot membrane such as nitrocellulose. A southern blot probe is then used to bind to a specific DNA fragment on the nitrocellulose. Southern blot analysis is performed on x-ray film by autoradiography. A southern blot protocol can be found online or from your professor if you are a college student. Southern blotting is used for determining who committed a crime by matching the supect's DNA to the DNA found at the crime scene. There are many applications of southern blotting. Whatman, G-Biosciences, and Pall supply products for southern blotting.

Tuesday, July 28, 2009

Clinical Centrifuges

Need a centrifuge for your veterinary lab or doctor's office? Then you need a clinical centrifuge. Searching for centrifuges can be daunting. Microscopes.com has a broad choice in basic clinical centrifuges. The first question is "What are you spinning?" If you are only spinning for blood separation then a fixed centrifuge speed is generally used. The Unico PowerSpin FX series will meet your needs. If you are spinning other samples in addition to blood such as urine, a variable speed centrifuge will be necessary. A good choice would be the Labnet Z100A or Spectrafuge 6C. Unico also offers the Powerspin LX Series. If you need to process more than 8 tubes the Unico Powerspin MX will spin up to 24 2ml to 15ml centrifuge tubes. If you require more options in centrifuge rotors and centrifuge speed the Labnet Z 706A will meet your needs at an affordable price. Spinning hematocrit tubes? Choose the Unico Powerspin BX. Many laboratories rely on the BD Clay Adams centrifuge for blood washing and other clinical applications.

Friday, July 24, 2009

Genomics

What is genomics? As you may have guessed, genomics is the study of genomes. Now we must define genome. A genome refers to a full set of chromosomes in a diploid organism and a single set of chromosomes in a haploid organism. You may have heard of the human genome project. The human genome project entailed locating all of the genes that make up the human genome. The human genome project was completed by a method called genome mapping. The human genome project started in 1990 and was completed in 2003. In the 1970s and 1990s Fred Sanger's research group was the first to sequence the complete genome of a virus and a mitochondrion. Since then the entire genome of many organisms has been mapped thanks to advances in technology. Companies like Whatman, Labconco, and Invitrogen supply products used for genomic and DNA research.

Thursday, July 23, 2009

Laboratory pH Electrodes

Use laboratory electrodes with pH meters to intermittently test the pH of a solution. Electrodes feature glass or epoxy bodies and come in either combination or half-cell styles. Laboratory electrodes are further categorized according to fermentation, general purpose laboratory,Specialty, and ion-selective applications. Fermentation electrodes resemble Industrial electrodes because they can be used continuously for several days. Fermentation electrodes are rugged, can be hooked up to some pH controllers, and are either autoclavable or in-situ-sterilizable. Beckman Coulter offers Nitrate, Flouride, Ammonia Gas Sensing, Chloride, Ammonium Ion-selective electrodes. There are a multitude of electrodes for your specific application. Here is a table to assist you in selecting the correct electrode for the correct application. *Biological samples, proteins, and tris buffer Calomel (Hg/Hg2Cl2) or double-junction *Pharmaceuticals Calomel (Hg/Hg2Cl2) or double-junction *Drinking water Standard Ag/AgCl with single-junction *Waste water Double-junction *Solutions with heavy metals Double-junction *Soil samples Soil electrode or double-junction *Fruits, cheese, and meat Spear-tip, Ag/AgCl *Flat surfaces, paper, skin Flat-surface

Wednesday, July 22, 2009

A Laboratory Instrument versus a Laboratory Aparatus

What is the difference between a laboratory instrument and a laboratory apparatus? An apparatus is a set of materials or equipment designed for a particular use. Laboratory equipment refers to the various tools and equipment used by scientists working in a laboratory. These include tools such as Bunsen burners, and microscopes as well as specialty equipment such as a humidity test chambers, Electroporators Hot Plates and Stirrers. Another important type of laboratory equipment is Laboratory glassware and plasticware

Tuesday, July 21, 2009

Silicone VS. Silicon

Silicone is a polymer incorporating the element Silicon, Si. The general formula is [R2SiO]n where R is an organic group and n represents the repeating nature of a polymer. R can be methyl, ethyl, phenyl... The properties of a silicone compound depends on the crosslinking between polymer chains. Silicone fluids have no crosslinking. Examples of fluids are Dow Corning silicone fluids for high and low temperature baths. Crosslinked silicone can range from a gel, to rubber, to a hard plastic. An example of silicone rubber is silicone pump tubing for peristaltic pumps and silicone clear stoppers. An example of silicone plastic is Saint Gobain Tygon tubing. Silicon is mostly found in silicon dioxide, SiO2. Silicon makes up around 26% of the earth's crust. Silicon is found right under carbon in the periodic table which means it has some of the same properties as carbon. For example silicon has semiconductor properties and it has the ability to combine with carbon hydrogen and oxygen producing silicone compounds. Glass is made from silicates, SiO2 minerals containing other metals such as borosilicate glass. Remember, don't confuse silicone with silicon they are related but not the same.

Friday, July 17, 2009

Genes

What is a gene? A gene holds the information necessary to produce cells within an organism. Genes are passed on to the next generation of offspring within a population. A gene contains the information required to make a protein, the building block of life. A gene is only a region of the genetic code (DNA) that has information for a specific protein. DNA can be compared to a blue print with a set of instructions. This set of instructions is known as gene regulation. Gene regulation determines when and where a specific gene is to be expressed. When a gene is expressed a protein is formed. The human gene that is expressed in liver cells may not be expressed in pancreatic cells. A gene mutation is a change in the nucleotide sequence in that specific gene. Gene mutations can be a cause of cancer. Many people refer to this as a "cancer gene" however there is no gene that causes cancer. One major focus of gene research is the connection between gene mutations and cancer. Companies like Promega, Eppendorf, and G-Biosciences provide products used in genomics research.

Tuesday, July 14, 2009

Are you a LABEXPERT?

Three are two definitions of a LABEXPERT that I know of; one is a person who works in a laboratory environment that is very knowledgeable in the their field of work. The other is a Brady LABEXPERT handheld laboratory labeling system. Anyone working in a laboratory environment could use a little help from both LABEXPERT's. The LABEXPERT that I want to focus on here is a handheld printer labeling system that will generate legible lables in a professional appearance for an assortment of lab sample containers. This printer already knows your labeling needs!It will also reduce the amount of time spent on labeling sample containers by hand. Because it is a personal size you can take it with you to different areas of your laboratory. Some of the best features of this printer include, an Auto-serialization feature, it can print 40 lables in less than 2 minutes, time and date stamping, built in bar coding, and Identilab software! This labeler will assist you in ensuring that your test tubes, vials, bottles and jars, even your laboratory notebooks all have a professional appearance!~Now get back to the things you really enjoy doing in the lab!

Friday, July 10, 2009

Protein Purification

Protein purification is a series of steps designed to isolate a protein of interest from a mixture of other substances. The purification of proteins is very important for understanding the structure, function, and interactions of the protein of interest. The process includes the separation of the protein of interest from all other proteins as well as other substances that may be binding to it. SDS-PAGE and protein purification often go hand in hand however SDS-PAGE only gives a rough estimate of the amount of a protein in a mixture. In order to successfully complete this process one must follow a protein purification protocol. A protein purification protocol will contain a detailed set of instructions that, when followed, will allow you to purify the protein of interest. Some protocols involve a protein purification tag. A protein purification tag can give a protein properties that it wouldn't normally have such as binding to a specific metal. Other protocols are specific to membrane protein purification. Several companies provide the necessary supplies for protein purification: Promega, Pierce, and Pall.

Tuesday, July 07, 2009

What is Cryogenics??

Cryogenics is the branch of physics that studies the phenomena that occur at very low temperatures. In physics, the production of low temperature systems; in biology, the use of such a system to preserve genetic material, especially in situations where the survival of a plant or animal species that would normally pass on that material in the course of reproduction is endangered. The main use of cryogenics is the fractional distillation of air to produce oxygen, nitrogen, and other gases. This process requires cooling the air to low temperatures to liquefy the gases in it. Natural gas, oxygen, nitrogen, and other gases are often liquefied for storage and transport because the liquid form occupy lesser space than the gaseous form. You might use a Nalge Nunc Dewar Flask or a Taylor Wharton Constant Temperature Refrigerator for the transport of your biological material. Cryogenic temperatures are also used in medicine to preserve certain biological substances, like bull semen (for breeding purposes), whole blood, and bone marrow. There are several uses of Liquefied Gases when dealing in the Cryogenics field. Liquefied gases are used as coolants in various industrial processes. Liquid nitrogen is used to freeze foods quickly. Liquid nitrogen in medicine, it is used to chill a needle like instrument called a cryoprobe. Cryoprobe is used by Doctors to freeze and destroy certain kinds of tissue, including warts, skin tumors, eye tumors etc.

Friday, July 03, 2009

Pipette

A pipette (also known as a pipet or a pipettor) is used for measuring liquids and dispensing them. If you work in a laboratory it is very important to learn how to pipette. Pipetting is a fundamental laboratory technique and is used in chemical and biological laboratories everywhere. Pipettes come in different designs and work by creating negative pressure in the area above the holding chamber and then releasing the pressure to expel the liquid. Pipets can be manually or electronically operated. Electronic pipettes come in handy for routine pipetting of a similar volume. A pipet aid is an electronic attachment that can be connected to various sizes of serological pipets. Pipetting is a skill and like any other skill, requires practice. Some laboratory techniques require very accurate pipetting so it's necessary to refine your skills. Companies like Thermo Fisher Scientific, Drummond, and Brandtech provide pipets and pipet supplies.