MicroscopesBlog: Expert's reviews, news, notes on Microscopes, Centrifuges, Lab Coats, Pipettes, Lab Safety, & more Lab Products!
Tuesday, December 29, 2009
Humidity! Can't live without it, but my hair can!
For all of us who have curly hair and want to keep it straight, humidity is on our naughty list. My hair can certainly live without it! How about you? Well, the world needs it, however some of our working environments either need it or don't. What you need to check it is an instrument called a Hygrometer. This is a device used to measure relative humidity in the air. The scientific world offers a selection depending on what your humidity issue is. Hygrometers are a delicate device and need to be calibrated for accuracy. Some industries that use hygrometers are, heating and air conditioning services, veterinarians, botanists, weathermen to name just a few. VWR, HB Instruments,
Control Company are the major players in the manufacturing of these instruments.Posted by Tammy Watkins at 1:39 AM Read Article 0 comments 

Friday, December 25, 2009
Retrovirus
Viruses come in many different shapes and sizes and use different methods of replication once they've infected the host cell. A retrovirus is a type of virus that uses an enzyme called reverse transcriptase to DNA from its RNA genome. The viral DNA then becomes incorporated into the host cell DNA and is known as a provirus. The retrovirus DNA is then passed on to the following generation. There are several characteristics common to all retroviruses: an envelope composed of a lipid bi-layer, dimer RNA, and proteins. Retroviruses have also been known to cause cancer. Retroviruses can cause proto-oncogenes to become incorporated in the proviral DNA. Labconco, Ika Works, and Nalge Nunc make products used in virology laboratories.
Tuesday, December 22, 2009
Is it Hot or Cold?
Lets talk laboratory thermometers, you need one right? Do you want a total immersion or partial immersion thermometer? Will you need Celsius or Fahrenheit Scale? You can get in White or Yellow Back, Mercury-Filled or Spirit filled. The choices are endless. Do you need an NIST Traceable Certificate? HB Instruments is one of the top manufacturers of laboratory thermometers Please remember when making a selection that Mercury is a regulated product, you may want to go with a spirit filled thermometer, less trouble and will provide you with the same results. Thermo Scientifc and ERTCO are also big manufactures of spirit filled thermometers. Red spirit filled is toluene kerosene or isoamyl benzoate and blue spirit filled is kerosene or isoamyl benzoate, and green spirit filled is kerosene.Friday, December 18, 2009
Black Death
You may hear the term "black death" or "plague" from time to time. What are people referring to. Between the years of 1347 to 1353 at least 33% of Europe's population died due to the black death, a bacterial infection caused by Yersina pestis. People think the world is coming to an end due to today's current events, imagine if one out of every three people you knew died. Europeans at this time thought that the world was about to end due to all of these deaths. Yersinia pestis is a gram-negative rod-shaped bacterium that can travel from animals to fleas to humans. This bacterium is a facultative anaerobe and belongs to the Enterobacteriaceae family. Mice carried to Europe on merchant ships were infected with this bacteria, fleas bit the mice. The fleas would then regurgitate the infected blood onto human skin. Once the human was infected the bacteria spread like wildfire throughout Europe. In September of 2009 a researcher at the University of Chicago was infected with this bacteria and eventually died. Labconco, Bel-Art, and EMD Chemicals all provide laboratory supplies used in Yersinia pestis research.
Tuesday, December 15, 2009
Lets Get Digital!
Okay, time to get prepared for your annual audits, many of you will need to have a refrigerator or freezer thermometer on hand that will monitor the temperature of your laboratory samples, vaccinations, or other such items. It will also need to come with a Traceable NIST Certificate, which is A serial numbered certificate is provided from an ISO 17025 calibration laboratory accredited by A2LA to indicate instrument traceability to standards provided by the National Institute of Standards and Technology. Some manufactures such as Control Company and brands such as VWR offer digital refrigerator/freezer thermometers such as these. Some come with an alarm that will sound when the temperature rises above or falls below set points.Monday, December 14, 2009
What Should I Look at With My New Microscope?
So you got a microscope for Christmas. Now what? Depends on your microscope. If you have a stereo microscope look at bugs. All bugs. Every bug you can find. Look at their wings, very different from bug to bug. Look at their eyes, their feet. Look at flowers. Every flower you can find. Look at the petals. Look at the stamen. Look at the anther. Look at the pollen. Freeze a slide and look at snow. Look at your hands. Look at jewelry. Look around your house and you'll find lots of things to put under a stereo microscope.
If you have a compound microscope do a swab of the inside of your cheek, put it on a slide and cover it with a cover glass. Look at the cells under the microscope. Put a drop of pond water on a slide and cover it with a cover glass. Look at the follicle of your hair. What about a fly wing? Freeze a slide and look at a snowflake, fast before it melts. Put some slides prepared already under the microscope. You'll never be done with stuff to look at under a microscope if you use your imagination.
Photo from microscopy-uk.org.uk
Friday, December 11, 2009
Bacteriophage
Viruses are some of the most complicated organisms known. They're so complicated that scientists don't know if they should be classified as living organisms. A bacteriophage is type of virus that infects bacteria and is roughly 20-200nm long. A bacteriophage may reproduce using a lytic cylce or lysogenic cycle. During the lytic cycle, the bacteriophage inserts its nucleic acid into the host cell and uses the host cell machinery to replicate. The host cell then bursts open or lyses and the virions are released. During the lysogenic cycle the viral genome incorporates itself into the host cell DNA. The host cell does not die immediately. The viral genome combines with the host cell DNA to form a prophage. Bacteriophages are some of the most widely studied viruses. Rockland Immunochemical, Emd Chemicals Inc, Himedia Laboratories all provide products used to study viruses and bacteria.
Tuesday, December 08, 2009
Do You Know The Difference Between Bacterial and Viral Infections?
What is the difference you ask? The answer is very simple. Bacterial Infections are caused by bacteria and Viral Infections are caused by a virus. Here are some examples of an infection caused by a bacteria; urinary tract, staph (Staphylococcus), toxic shock syndrome, and Salmonella to mention a few. Here are some examples of diseases caused by a virus; influenza, German measles, mumps, aids, rabies. Bacteria are single celled microorganisms that live in many different types of environments. A mixture of those environments can include extreme heat or cold while other bacteria live in human intestines where they assist in the digestion of food. Most bacteria is not harmful to to humans. Viruses are tinier than bacteria and require a living host, such as plants or animals to reproduce. Otherwise they cannot survive. When a virus enters your body it grows rapidly in some of your cells and takes over the cell mechanisms. Changing its focus to produce the virus. Viruses need to be looked at using an electron microscope and bacteria can be viewed under a
brightfield microscope. Nikon, Unico, LOMO are some of the most common microscopes used in the microbiology world today.Monday, December 07, 2009
Can I See Atoms With My Microscope?
What we can see is limited by light waves. Visible light waves are around 380 to 750 nanometers
(0.0000380 cm). This seems very small but compared to an atom light waves are HUGE. We are able to see because light waves bounce off of an object and enter our eye. The light waves have to be considerably smaller than the object they bounce from in order to convey detail. The radius of an atom is less than 1/1000 the length of a visible light wave. Even the largest molecules - proteins - have a diameter of only around 5nm. Viruses range from 10nm to 450nm so they also can not be seen under light microscope. Some of a cell's organelles have a diameter of around 2000nm or 2um (micrometers). These are some of the smallest structures which can be seen with the light microscope. With a Nikon E200 microscope using the 100x objective (1000x magnification) an organelle would only appear to be ~2mm.
Friday, December 04, 2009
Fungi
A fungus is a eukaryotic organism and is part of a large kingdom of organisms that includes yeasts and molds. Fungi differ from bacteria and other microorganisms because of the main component of their cell walls, chitin. The division of biology devoted to the study of Fungi is known as mycology. Mushrooms are one of the most well known members of the fungi kingdom. Fungi have an integral role in the decomposition of organic matter and aid in nutrient recycling. Fungi are also responsible for fermenting wine, beer, and soy sauce. many species of fungi produce antibtiotics such as penicillin. This naturally occuring chemical has a narrow spectrum of biological activity however after slight modifications this drug has a broad spectrum of activity. The ability of Fungi to produce antibiotics has improved the quality of our lives and will continue to play an important role in anti-microbial drug research. Bel-Art, Axygen, and Greiner Bio-One all provide products used to research species of the kingdom Fungi.
Tuesday, December 01, 2009
Bacteria, Fruit, and Pesticides
Did you know that pesticides can help bacteria grow on fruits and vegetables? Some scientists say that pesticides actually support the growth of life threatening bacteria on fruit and vegetable crops. While reading on of the journals from New Scientist Magazine, I found that because of this bugs like Salmonella, E. coli and Shigella could pose a threat to people eating raw fruit and vegetables. The problem starts not with the pesticides themselves, but the water used to mix them. There is an alarming rise in cases of food poisoning caused by fresh produce, so set out to determine if contaminated pesticide sprays were to blame. Pesticides are generally purchased in concentrated form and mixed with water. University of Manitoba researchers found that "In most cases the water that the farmers are going to use is whatever is available," "If you take a look at what happened with Ontario, with Walkerton, that water's not only used for drinking but it's also used for irrigation." Six people died after drinking E. coli contaminated water in the town in the year 2000. The researchers mixed a variety of common pesticides, herbicides and fungicides with water contaminated with pathogens like Salmonella or E. coli. Some killed the bugs quickly. But in about one-third of the pesticides, the bacteria actually flourished. "Numbers could increase 1,000-fold," Blank said. Salmonella, E. coli and Shigella grew best, particularly in the fungicide chlorothalonil. "If it's spread on a crop that's already standing, then you're contaminating the crop," he said, adding that "many of these fruits or vegetables ... are usually consumed raw or with minimal cooking." Watering fruit and vegetable crops with contaminated water also poses a threat. But the health risks are greater if that water is mixed with pesticides that foster bacteria growth. Please take heed and wash all your fruits and vegetables before consuming. Scientists in Manitoba used products such as petri dishes, microscopes, pipettors to perform their research and look at the data before publishing their findings in the New Scientist Magazine.