MicroscopesBlog: Expert's reviews, news, notes on Microscopes, Centrifuges, Lab Coats, Pipettes, Lab Safety, & more Lab Products!

Friday, January 29, 2010

Helminths

Helminths are multicellular eukaryotic animals. Many are parasitic. Helminths can be dioecious (male and female individuals) while some are hermaphroditic (having male and female reproductive organs). The definitive adult harbors the sexually mature adult helminth while intermediate hosts may be necessary for developmental stages. Platyhelminths or flatworms are flattened from front to back. The digestive system is incomplete as there is an opening for food to enter and an opening for waste to exit. Trematodes or flukes have flat leaf shaped bodies with a ventral sucker and an oral sucker to hold them in place and suck fluids from the host. They also obtain food by absorption through their cuticle. Cestodes or tapeworms are intestinal parasites. The nematodes or roundworms are cylindrical and tapered at the ends. They have a complete digestive system and many human parasites are found in this group. LOMO, Konus, and Greiner Bio-One make products used in parasite research.

Tuesday, January 26, 2010

Pirates Walk the Plank in Style!

I'm not talking about pirates; however I am going to talk a little bit about Plankton, that can be found in the Ocean. Plankton is carried through the ocean through the current. Plankton consists of various microscopic creatures. Did you know that Plankton is the most prevalent life and food source in the ocean? Phytoplankton, which carries on photosynthesis near the ocean surface, serves as food for the zooplankton and fish. Plankton are microscopic animals (zooplankton) and algae (phytoplankton) that live in the ocean. They drift on currents and provide food for many ocean residents. Get this, A mouthful of seawater may contain millions of bacterial cells, hundreds of thousands of phytoplankton and tens of thousands of zooplankton. Yuk! Don't want to swallow ocean water; however you may want to explore just a drop of it under a microscope. What you will find are amazing, super small, microscopic living sea plants and creatures. Magnify your sample of ocean water by 25X and you will be amazed of the living plants and animals, residents of the ocean that you will be able to see. You will need a compound microscope, some microscope slides and microscope cover slips to view your sample properly. If you want to work in the field, right by the ocean, you could use a Nikon Mini Field Microscope, perfect for on the go field work! It's water resistant and has a 20X magnification, it's compact and lightweight. A perfect microscope for the vacationer. For the beginner, I would suggest the Unico brand microscope. Unico offers an M16 a perfect microscope for the youngster interested in a hobby using microscopes to view parts of the world they cannot begin to imagine exist through the naked eye alone! Good Luck Beginners! Have a wonderful time learning new things about our oceans and the species within.

Friday, January 22, 2010

Algae

Algae are photosynthetic autotrophs, they use light to convert atmospheric carbon dioxide into carbohydrates for energy. The body of the multi-cellular alga is a thallus, which may collectively function as holdfasts to anchor them. They often have stemlike stipes and leaflike blades. Algae also have gas filled bladders. There are different types of algae. Brown algae may be 50 meters in length. Red algae are branched and multicellular and live at greater depths than do other algae. Dinoflagellates are unicellular algae and are referred to as plankton because they are free floating. Some produce neurotoxins that are ingested by shellfish and can eventually poison humans. Algin is a food thickener and used in the production of goods and is extracted from the cell walls of algae. A large increase in plankton is known as a bloom. Blooms of dinoflagellates are known as red tides. G-Biosciences, Himedia Laboratories, and Agilent Technologies supply products used in algae research.

Tuesday, January 19, 2010

A little history about Forensics

Ok, this topic interests me a lot. Did you know that in the history of Forensic Science dates back thousands of years? Fingerprinting was one of it's first applications. n 1835, Scotland Yard's Henry Goddard became the first person to use physical analysis to connect a bullet to the murder weapon. n 1836, a Scottish chemist named James Marsh developed a chemical test to detect arsenic. Nearly a century later, in 1930, scientist Karl Landsteiner won the Nobel Prize for classifying human blood into its various groups. Other tests were developed in the mid-1900s to analyze saliva, semen and other body fluids as well as to make blood tests more precise. So what types of laboratory products do Forensic labs use? While much of the supplies are consumables; for instance swabs, for evidence collection, sterile petri dishes for sample collection, disposable exam gloves, evidence collection jars, sterile sample containers, forceps, and lab coats. The list could go on and on. The list above consists of consumable Forensic laboratory products. While the Forensic lab uses many consumable products, we also have to look at other products the Forensic Scientist might use such as durable laboratory products, centrifuges for spinning samples, pipettes for drawing the samples and placing into centrifuge tubes that are then placed in the centrifuge, and last but not least microscopes.

Monday, January 18, 2010

Glass Microfiber Filter

GMF Filters, like cellulose filters are depth filters; filtration is accomplished throughout the filter, not just the surface. Glass microfiber filters are used when a high filtration efficiency is required and a high loading capacity is needed. GMF filters have particle retentions lower than cellulose filters, down to 0.7um. Not only are glass microfibers used to filter fluids, they are used in air sampling equipment for collection of particulates and aerosols. Some GMF filters are designed to be heated to up to 550 deg for ignition of the sample in gravimetric determinations. Other applications include: liquid scintillation counting, radio immunoassay, analysis of water, analysis of wastewater, and filtration of hot gases and liquids. Quartz (SiO2) MF filters are used for air sampling in acidic gases, stacks, flues and aerosols, particularly at high temperatures. Whatman and Ahlstrom are two manufacturers of glass microfiber filters.
Photo from Alliant Energy

Friday, January 15, 2010

Protozoa

Protozoa are on-celled eukaryotic organsims that mostly feed on bacteria and small particulate nutrients. They are classified by their means of motility. Protozoa reproduce asexually by fission, budding, or schizogony. Schizogony involves the fission of the nucleus prior to cell division. Some protozoa produce gametes or gametocytes-haploid sex cells that fuse to form a zygote. Most protozoa are aerobic heterotrophs, but most intestinal protozoa are capable of anaerobic metabolism. A few, like Euglena, contain chlorophyll and are photoautotrophs. Some protozoa absorb food through the cell membrane, but many have a coating, the pellicle, that does not allow them to do so. Amoebas engulf food and phagocytize it. Digestion in all protozoa takes place in the vacuoles, and waste is eliminated through a pore. BD, Nikon, and Axygen supply products used in protozoa research.

Tuesday, January 12, 2010

Fat Cells

What is a fat cell? Adipocytes, which is a connective tissue that has also known as lipocytes and fat cells, are the cells that primarily compose adipose tissue, specialized in the manufacturing and storing of fat. There are two types of adipose tissue, white tissue and brown adipose tissue, which are also known as white fat and brown fat, respectively, and comprise two types of fat cells. The adipocyte is essential for the body to maintain energy in balance, storing calories in the form of lipids. Under the microscope the adipocyte appears swollen with triglycerides. The nucleus is shifted to one side by the fat. The cytoplasm of the cell looks like a thin line surrounding the pool of fat. Cytoplasm is the substance that fills the cell. It is a jelly like material. You can view fat cells under several types of microscopes using several different kinds of applications. Brightfield and Phase Contrast are two ways you can view live cells under the microscope. Using a fluorescent microscope you can stain your fat cell. The dye or stain that you use will bind to specific molecules and will reveal their location when viewed under a fluorescence microscope.

Monday, January 11, 2010

Cellulose Filter Paper

One of the most common items in the lab is the ever-present cellulose filter paper. Filter papers are depth filters - they retain particles within the filter matrix not just on the surface like membrane filters. Not all filter paper is the same. There are great differences depending on the application. What is important, the filtrate or the residue? If the fluid coming through the filter paper is more important a qualitative filter paper would be sufficient. If the sample retained by the filter paper must be quantified, a quantitative filter paper must be used. The paper may be dried and burned in a crucible as for heavy metals testing. In this case a low ash or ashless filter would be needed. Some filtration requires retaining particles larger than say 2um and allowing the rest to go with the filtrate. Filter paper grade is determined by the size of particle retention. Filter paper is also graded by the speed at which an aqueous solution will pass through. The smaller the particle retention the slower the speed.
The support for filter paper can be a cone shaped funnel; in this case the paper would be fluted. With a a fluted filter and cone funnel gravity is allowed to carry the sample through the filter. Another type of funnel, Buchner is used with the filter flat over a perforated support. In this case a vacuum flask with a side arm is used to contain the filtrate. The vacuum pulls the sample through the filter.
Whatman filter papers are designed for many uses from soil analysis to malt and beer filters. Whatman's website is very helpful in choosing the right filter paper for your application. Ahlstrom paper is also available in many grades
Image from Bezirksverband der Gartenfreunde Heidelberg e.V.
Image from web.centre.edu/

Friday, January 08, 2010

Adenovirus

An adenovirus is a medium sized virus in the range of 90-100nm. This virus is non-enveloped and has a double stranded linear DNA genome. The strand of DNA is between 26 and 45 Kbp allowing it to have theoretically, between 22 to 40 genes. There are 53 serotypes or versions of this virus in humans which are responsible for 5-10% of upper respiratory infections. The virus got its name because it was originally isolated from human adenoids. The adenovirus has a unique spike or fiber on the capsid that allows the virus to attach to the host cell. Adenoviruses are resistant to chemical and physical agents as well as adverse ph conditions. This characteristic allows them to survive for extended periods of time outside of the human body. In the past U.S. Military recruits were vaccinated against two serotypes of the adenovirus. There are currently no vaccines against the adenovirus and the best way to prevent a viral infection is by washing your hands routinely. Labconco, BD, and Eppendorf supply products used in virology or the study of viruses.

Thursday, January 07, 2010

Chromatic Aberration

Microscope optical systems suffer from the same defects as all other lens systems. Among these are various forms of chromatic aberration and spherical aberration. Chromatic aberration is the failure of a lens or a lens system to focus all colors at the same focal point. All modern microscope designs go to great lengths to to minimize or eliminate this defect. It is commonly accomplished by designing lenses that are defined as being achromatic. Achromatic means "without color" and is usually meant to imply without color distortion. This means that the objectives bring images into focus without dispersing their colored components. In simple achromatic microscope objectives the correction is made so that the red and blue wavelengths are brought to a single point of focus. This correction helps to eliminate chromatic defects for most of the visible spectrum in a very effective and cost efficient manner. Manufacturers who work very hard to achieve this include NIKON, UNICO and MOTIC. When searching for a quality microscope, achromatic optics should be high on the list of requirements.

Wednesday, January 06, 2010

The secret to enjoying astronomy

Is there a secret to enjoying amateur astronomy?

Is it all about buying the right telescope or maybe a buying astronomical binoculars? Is it all about getting the right telescope accessories? Maybe it’s getting the right astronomy book or the latest astronomy software. Maybe …

All those things do help, no doubt about it, but the secret of enjoying amateur astronomy is not about telescopes, binocular or telescope accessories; it’s all about perspective. Think of it this way. How many people, even in our high tech ultramodern society, have ever seen Saturn and its rings? No, I’m not talking about seeing a picture of Saturn in a magazine or a book or seeing Saturn on TV or an astronomy video. I’m talking about seeing Saturn, firsthand and personally, with their own eye, through a telescope. The answer? Only a very small percentage of our population can claim they actually saw Saturn and its rings, rather than a picture of Saturn and its rings. To be sure, when we amateurs look at Saturn through a telescope, it does not match up with the splendor of those pictures, but bragging rights go to those who actually saw Saturn for themselves. When you simply look at a picture, you never go beyond spectator status; when you search with your telescope and find Saturn and then see it in the eyepiece of your telescope, you cross the line from spectator to explorer. Now, that’s perspective.

Perspective? Nothing exemplifies perspective like seeing galaxies through a telescope. Most galaxies in a telescope appear as fairly dim glows or grey smudges – not much detail can be seen in even a large amateur telescope. Visually, they lag far, far behind pictures you see in a book or magazine. So why do so many amateur astronomers love to look at galaxies? You guessed it – perspective. When you realize that the smudge in your eyepiece is really its own universe, containing many billions of stars, your imagination soars. Is there intelligent life in that galaxy? Is their some intelligent being look back at out galaxy, as we are looking at theirs? Even more incredible is the fact that those galaxies are immensely distant. Even at the speed of light, it takes light many millions of years to deliver that image to the eyepiece in our telescope. What you see in the eyepiece is the galaxy as it was many millions of years, ago. Yes, your telescope is your own personal time machine for looking back into the past.

It' all about perspective.

Tuesday, January 05, 2010

Boy You're Sharp!

Im not talking about your witt or mind! I'm talking about knives for the laboratory; laboratory knife sets If you are looking for the perfect knife set for general cutting of tubing, corks, plant samples, or paraffin then here is the set to get. It is made by Control Company. The set includes anything from ultra-micro to heavy duty cutting edges! The blades are manufactured with high standard grade steel and ground to razor sharpness for superior accuracy in cutting. You have probably heard of the manufacturer X-ACTO, they have been a household and industrial name for years. They also have a knife set available for lighter cutting applications, such as paper, wood, plastic, cloth, and foam board. This would be a great set for the hobbiest in your life!

Monday, January 04, 2010

Magnification Microscope

Magnification can mean a lot of things. In the case of an optical microscope it means magnification of the optics image. We all have seen magnified images that don't tell us anything new about the image. The object is bigger but either blurry or pixelated. The key here is resolving power. A good light microscope will magnify the image and reveal new sharp details which are not visible at lower magnifications. Optical magnification up to approximately 400X showing a resolved image with good edge definition can be achieved with a reasonably priced microscope (~$500). Higher magnifications require very good optics, very good illumination, good light control (condenser) and perhaps an oil objective.
When a camera is added to a microscope the magnification of the microscope's eyepiece (eg. 10X) is lost. Magnification beyond the optical magnification of the microscope objective (eg. 40X) is then dependent on the electronic magnification of the camera and the size of the computer screen. Electronic magnification is dependent on the resolution of the microscopes camera. If you are projecting to a 19" computer screen a 1.2 megapixel camera is sufficient as that is the typical number of pixels on the computer screen. If you wish to project to a larger screen or you wish to produce photographs a camera with more resolution is desirable.
Microscope systems may claim magnifications greater than 1000X but the images will not be well resolved. In a large number of cases lower magnifications can give you more information about your sample, especially if you are projecting the image on to a computer screen.
Image from Iowa State University Image Gallery - Pond Scum, 500X

Friday, January 01, 2010

Prion

Most people haven't heard of the word prion however they have heard of mad cow disease. This disease along with Creutzfeldt-Jakob disease (CJD) in humans is caused by a prion, an infectious particle made mostly of protein. A prion is a misfolded form of the PrP protein. Prions do not replicate, they cause other PrP proteins to become misfolded. Prions cause proteins to form into an amyloid, or a tightly packed beta sheet. These packed beta sheets are extremely dense and very stable. When they accumulate they become extremely resistant to denaturation and chemical treatment. Prions accumulate extracellularly within the central nervous system. Diseases caused by prions are untreatable and fatal. Researchers are scrambling to find answers to the unanswered questions about diseases caused by prions. EMD, Abgent, and G-Biosciences provide products used in prion-based research.