Wednesday, January 23, 2008
Pipetting and Liquid Handling
A big part of working in most research labs these days has to do with pipetting. Whether one has to transfer acids, bases, DNA, or aqueous solutions, using different types of pipettes is a daily occurence in the lab. microscopes.com has a plethora of different pipetting devices to help your everyday needs. We carry serological pipets, transfer pipets, volumetric pipets, pipettors, burets, syringes, and many more liquid transfer devices. We are constantly adding new brands such as Axygen, BD, Barnstead, Heathrow, Kimble/Kontes, Labcon, Nalge Nunc, Sorensen, and Wheaton to our website. When transferring liquids, always use proper lab technique in order to conserve product. If transferring several different liquids, please be aware that small mistakes in volume can add up to big mistakes overall and affect your data and the results of your studies. Don't hesitate to contact the laboratory technical specialists on-site for any help with application questions.
Posted by Yury P at 8:26 AM Read Article 0 comments 

Friday, January 04, 2008
How To Choose the Right Spectrophotometer
Whether one is using a spectrophotometer for sample analysis in the visible range or in the ultraviolet range, it is important to know what you plan to do with your reader before purchasing one. Microscopes.com has many spectrophotometers for sale by Thermo, Beckman, and Unico. The ones we currently carry are intended for UV and visible work. The first thing that we would need to know is what wavelength is needed to be read. Anything below about 280-300nm is considered in the UV light range. Most visible specs usually read in the 400-1100 nanometer range, however. It is also important to know how many samples you intend to read in one session. Many current spectrophotometers currently have multi-sample platforms which allows you to read one 'blank' and several samples in a row, without removing your cuvettes from the photometer. Speaking of which, it's extremely important to know what type of cuvette you need for your application. Readings below 250nm require a quartz cuvette. Reading in the upper UV range, however, (260-340nm) can use special plastic cuvettes with UV windows. Readings in the visible range can use disposable cuvettes that are usually very inexpensive to buy.
Wednesday, January 02, 2008
Snowflakes Under The Microscope
With the New Year upon us, try to take a moment or two today to coax a few secrets from snowflakes that fell on Monday. A cheap magnifying glass gets you part of the way. A microscope gets you even closer to pulling back the curtain on delicate, complex formations that are as fleeting as they are stunning. Getting a sense of their beauty sometimes requires a closer look and an understanding of what you're seeing. The easiest to spot with the naked eye is the "stellar dendrite," whose six symmetrical arms can feature elaborate branches and designs worthy of holiday stationery. But not every snowflake is a perfectly formed, asterisk-shaped wonder. Some are needlelike, and others are columns or plates. Still others are globular, sandlike blobs or flecked with bits of pollution. All of them have endured a harrowing journey through the atmosphere that makes them unique. Each flake is a collection of crystals, which start out as a tiny droplet of water in a cloud high in the Earth's atmosphere. When it freezes, the particle of ice grows into a six-sided plate because of the way hydrogen and oxygen molecules bond. As it grows, branches spring from the corners. The more water vapor condenses on the crystal, the more those arms grow. Its earliest moments, though, are pretty rough. Snowflakes are bashed and battered as they tumble toward the ground, changing shapes rapidly as they pass through different temperatures and humidity. So what are that chances that any two are exactly alike? Pretty darned small. Even though some may look similar, snowflakes hide a staggering array of possible arrangements - a tiny jagged branch here, a bulbed tip there and on and on. It's unlikely that any two complex snowflakes in the planet's history have ever been exactly the same. That's part of the magic of pausing to watch them fall and taking a closer look. With over 6 billion people on the planet, surely a few of us can be spared to ponder the subtle mysteries of snowflakes.
Friday, December 07, 2007
Safety Goggles and Safety Glasses - Which Do You Use?
We are currently trying to optimize our offering of Safety Glasses on microscopes.com and would like your input. Some of you have been extremely helpful when I asked for your help before and I'm hoping you can help me again. We would like to know which brands or types of safety goggles and safety glasses you use in your laboratory. Currently, we have a limited offering from the makes of Uvex, Bacou-Dalloz, Aero, Allsafe, US Safety, Smith & Wesson, Howard Leight, Kimberly Clark, Nalge Nunc, Crews, Dewalt and Bolle. Safety in the laboratory is of the utmost importance to everyone's health, so face protection is needed weather one is working with chemicals, in the lab, or with many industrial applications. We have to prioritize the manufacturers that we work with and would appreciate any input you can provide. The best product suggestions will receive a FREE pair of safety glasses!
Wednesday, December 05, 2007
Microplate Instrumentation
Our sales have been increasing steadily. Thank you to everyone who has been purchasing all the laboratory equipment and lab consumables that we carry. Some items that I've been getting questions on lately has been microplate instrumentation. We carry several brands of microplate readers including Beckman Coulter, Turner Biosystems, Thermo Electron, and many others. These include microplate photometers that read in the UV and Visible ranges. We also carry Fluorometers and Luminometers that read fluorescence and luminescence, accordingly. When you use these readers, please don't forget to purchase the correct microplates for your particular experiment. Photometers use quartz or UV plates to read such things as DNA or RNA. You can use regular polypropylene microplates for visible readings. Black plates are used for fluoresence to cut down on the amount of crosstalk in microwells. White plates are best for luminescence, which normally gives such a weak signal, that white plates are the only ones that can maximize the signal given off from the microplate reader. In the last few years, there has been a rise in the number of multifunction readers being manufactured. These would read a combinations of absorbance, fluorescence and luminescense. Other experiments that can be now done are enzymatic kinetics reactions, TRF, and many other experiments.
